| 摘要 |
A biosensor chip that is fitted with a reaction chamber of small volume capable of measuring with traces of measuring sample and has a potassium ferricyanide of minute crystal grain diameter disposed in the reaction chamber so as to attain rapid and accurate measuring; and a process for producing the same. Biosensor chip (1) has electrodes (3,4) disposed on top of lower substrate (2) and further has lower spacer (5(7,8)) bonded thereto. Upper spacer (11(12,13)) is bonded to upper substrate (15), and the upper and lower spacers (5,11) are bonded together with adhesive (10). Lower groove part (9) is provided between long and short lower spacers (7,8). Upper groove part (14) is provided between long and short upper spacers (12,13). The upper and lower groove parts (14,9) define reaction chamber (17). The volume of the reaction chamber (17) is 0.3 VL, and enzyme (18) and potassium ferricyanide are disposed in facing relationship with a spacing therebetween. The crystal grain diameter of potassium ferricyanide is not greater than 100 mum, and the amount thereof is Vx0.1 mg wherein V is the volume of the reaction chamber (17).
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