THERMOSTABLE PULLULANASES

摘要

2088592 9202614 PCTABS00010 This invention is within the field of thermostable enzymes. More specifically, the present invention relates to novel thermostable pullulanases obtainable from members of the genus Pyrococcus and to processes for the preparation of these enzymes. The invention also relates to recombinantly produced pullulanase preparations consisting essentially of a homogeneous pullulanase component, the DNA encoding the pullulanase being derived from the genome of a member of the genus Pyrococcus. Moreover, the invention relates to high expression processes for preparation of the pullulanase components devoid of any contaminating enzyme activities. The invention further relates to the use of the pullulanases in starch converting processes, and to liquefaction and/or saccharification processes.