TRANSFORMED EMBRYOGENIC MICROSPORES FOR THE GENERATION OF FERTILE HOMOZYGOUS PLANTS

摘要

The invention relates to transformed, embryogenic microspores and progeny thereof characterized by being transformed by Agrobacterium tumefaciens, capable of leading to non-chimeric transformed haploid or doubled haploid embryos that develop into fertile homozygous plants within one generation and containing stably integrated int o their genome a foreign DNA, said DNA being characterized in that it comprises at least one gene of interest and at least base pairs within the right border sequence of Agrobacterium T-DNA. The invention furthermore relates to a method for the incorporation of foreign D NA into chromosomes of microspores comprising the following steps: a) infecting of embryogenic microspores with Agrobacteria, which contain plasmids carrying a gene of interest under regulatory control of initiation and termination regions bordered by at least one T-DNA border, b) washing out and killing the Agrobacteria after co-cultivation.