Method for concentrating sample constituents and for multiplying nucleic acids from a biological sample which are contained in the sample constituents

摘要

A method is disclosed for concentrating sample constituents and for multiplying nucleic acids from a biological sample which are containing in the sample constituents. The nucleic acids are amplified on the same filter on which the sample constituents are also separated off.

主权项

1. A method for concentrating sample constituents and for multiplying nucleic acids from a biological sample which are contained in the sample constituents, comprising:
1) providing a biological sample in liquid form; 2) introducing the sample into a separating system having at least one filter; 3) accumulating the sample constituents contained in the sample by separating off the sample constituents on the filter; 4) adding amplification reagents to the separating system; and 5) directly amplifying at least one nucleic acid present in the sample constituents; wherein the amplification in step 5) is carried out by PCR and comprises at least: 5.1) extracting nucleic acids without using chemical lysing agents and denaturing the at least one nucleic acid; 5.2) annealing primers; and 5.3) extending primers.