| 摘要 |
<p>Crude Florunner extracts were fractionated by anion-exchange chromatography using a step gradient. A protein peak which eluted at 10 % NaCl and demonstrated intense IgE-binding was further analyzed by two-dimensional SDS- PAGE/immunoblot analysis. The majority of this fraction is a protein which has a molecular weight of 17 kD and a pI of 5.2. Sequencing data from the N-terminus revealed the following initial 9 amino acids: (*)-Q-Q-(*)-E-L-Q-D-L. Based on IgE-binding activity and no known amino acid sequence identity to other allergens, this allergen is designated Ara h II. Ara h II may be used to detect and quantify peanut allergens in foodstuffs. Serum IgE from patients with documented peanut hypersensitivity reactions and a peanut cDNA expression library were used to identify clones that encode peanut allergens. One of the major peanut allergens, Ara h I, was selected from these clones using Ara h I specific oligo-nucleotides and polymerase chain reaction technology.</p> |
