A MODIFIED PEPTIDE AS AN ANTICANCER AGENT.

摘要

A novel modified peptide AT-01 has been synthesized from the microbiomic secretory protein MPT63 of Mycobacterium tuberculosis. This peptide (about 30 amino acids) contains a portion of the immunogenic region of MPT63 (131 amino acids). It has been found to stabilize SMAR1 (Scaffold Matrix Attachment Region Binding Protein 1), a tumor suppressor protein which is well characterized as anti-cancer. In subsequent reasearch, AT-01 was modified further to generate 6 new, independent peptides. Both ITC and docking studies supported the interaction between the various peptides and full-length/ Protein Binding Domain of SMAR1. Among the peptides AT-01C and AT-01D were found to be more effective than the other peptides. Hence, we have used AT-01C and AT-01D for the further experiments. AT-01D also attenuated cell migration of MDA-MB231 cells which supports the anti-metastatic activity. Inhibition of the cell growth from the Colony Formation Assays too supported its anticancer activity. It does not cause toxicity to the cells at high doses which demonstrates great potential value as a safe and effective cancer therapy.