| 摘要 |
PCT No. PCT/SE89/00232 Sec. 371 Date Dec. 26, 1990 Sec. 102(e) Date Dec. 26, 1990 PCT Filed Apr. 26, 1989 PCT Pub. No. WO89/10398 PCT Pub. Date Nov. 2, 1989.A platelet lysate is produced from plasma derived from animal whole blood that contains an added citrate to prevent coagulation of the blood during storage. The lysate is prepared by centrifuging plasma to produce a platelet rich paste, adding calcium to the paste to lyse the platelets therein and coagulate fibrinogen to produce a clear liquid containing lysed platelets, sterile filtering the liquid and collecting a liquid filtrate containing the lysed platelets. The obtained lysate of platelets can be used to wholly or partly replace foetal calf serum in cell culture, such as in culture of hybridoma cells for preparing monoclonal antibodies. |
