METHODS FOR THE ENZYMATIC ASSEMBLY OF POLYNUCLEOTIDES AND IDENTIFICATION OF POLYNUCLEOTIDES HAVING DESIRED CHARACTERISTICS

摘要

The present invention provides methods of preparing large polynucleotides of arbitrary sequence and in a manner that will readily lend itself to automation. The present invention provides methods of preparing a polynucleotide having at least 200 nucleotides in either a 5' to 3' or 3' to 5' direction by ligating a plurality of oligonucleotides, the assembly of which, represents the nucleotide sequence of the desired polynucleotide. The present invention also provides libraries of polynucleotides and screening of libraries for polynucleotide members having desired properties.