Systems and methods for performing online extracorporeal photopheresis

摘要

Systems and methods for performing online extracorporeal photopheresis of mononuclear cells are disclosed. Whole blood is removed from a patient and introduced through a processing set into a separation chamber to separate the desired cell population from the blood. The separated cell population is processed through the set which is associated with a treatment chamber where the cells are treated. Once treated, the cells are returned to the patient. The processing set remains connected to the patient during the entire ECP treatment procedure and provides an online, sterile closed pathway between the separation chamber and the treatment chamber.

主权项

1. An online extracorporeal photopheresis system comprising:
(a) a disposable fluid circuit comprising:
i. a processing chamber for separating whole blood into one or more components including mononuclear cells,ii. at least one storage container adapted to receive mononuclear cells wherein at least a portion of said container is transparent to light of a selected wavelength, (b) a freestanding separation device having said processing chamber received therein for effecting separation of said mononuclear cells from whole blood, (c) a freestanding irradiation device independent of, and spaced apart and housed separately from, said separation device and having said mononuclear cell storage container received therein simultaneously with the processing chamber being received in the separation device for treating said mononuclear cells with a selected dose of light, (d) a controller separate from the separation device and the irradiation device for communicating with and controlling both devices, the controller configured to i. dilute the mononuclear cells received in the storage container with a diluting solution comprising saline or plasma prior to activation of the irradiation device, ii. concentrate the irradiated diluted mononuclear cells by separating supernatant liquid, iii. combine the concentrated mononuclear cells with a wash solution, and iv. separate the combination into a concentrated washed cell product and supernatant liquid, (e) wherein said disposable fluid circuit provides a sterile closed pathway between said processing chamber when mounted in said separation device and said storage container when mounted in said irradiation device.