PURIFICATION OF AN AMIDE FORMING ENZYME

摘要

A method for preparing an amidating enzyme product from a solution of proteins containing the amidating enzyme comprising a heat treatment step. The solution of proteins may be derived from mammalian tissue, such as porcine pituitary gland tissue. The method may be used to enhance the specific activity of the amidating enzyme product and/or especially to purify the amidating enzyme from a solution of proteins. Optimum conditions for heat treatment are a temperature of about 60C for a duration of from 5 to 10 minutes.