Oncogene identification by transformation of rk3e cells and uses thereof

摘要

The present invention is directed to methods of identifying new carcinoma oncogenes or analyzing functions of known carcinoma oncogenes by transformation of RK3E cells. Also provided are methods of identifying oncogene-specificity of known drugs or screening for new drugs that inhibit oncogenes activated in carcinoma by utilizing RK3E cells. Further provided are methods of identifying alterations in cellular enzyme, protein, or mRNA levels or activities by utilizing RK3E and oncogene-transformed derivatives. Still further provided are a novel oncogene GKLF with cDNA sequence and amino acid sequence for the protein, and applications of such gene/protein in medical diagnosis and treatment.