Method for the production of polypeptides

摘要

New methods for the production of recombinant polypeptides from inclusion bodies are disclosed. Modulation of the cell culture conditions positively affects the yield of the recombinant polypeptide in active form. In one aspect, the methods comprise (a) cultivating a host cell at a first temperature, the host cell comprising a nucleic acid encoding a recombinant polypeptide, (b) lowering the cultivation temperature from the first temperature to a second temperature, and (c) cultivating the host cell at the second temperature.

主权项

1. A method for the production of a recombinant polypeptide in inclusion bodies, the method comprising
(a) cultivating a bacterial host cell at a first temperature, the host cell comprising a nucleic acid encoding said recombinant polypeptide, (b) lowering the cultivation temperature from the first temperature to a second temperature, and (c) cultivating the bacterial host cell at the second temperature,wherein lowering of the temperature is performed when the cell culture has reached an optical density at 600 nm of between 10 and 50.