Nucleic acid detecting or quantifying processes

摘要

This invention provides novel compositions and processes for analyte detection, quantification and amplification. Nucleic acid arrays and libraries of analytes are usefully incorporated into such compositions and processes. Universal detection elements, signaling entities and the like are employed to detect and if necessary or desirable, to quantify analytes. Amplification of target analytes are also provided by the compositions and processes of this invention.

主权项

1. A process for detecting or quantifying non-nucleic analytes of interest, said process comprising the steps of:
1) providing:
a) a sample comprising analytes, said analytes of the sample comprising or suspected of comprising one or more of a first non-nucleic acid analyte of interest and a second non-nucleic acid analyte of interest, wherein
said non-nucleic acid analytes comprise oligopeptides, polypeptides, oligosaccharides, polysaccharides, lipids, ligands, or combinations thereof;b) an array on a solid surface, said array comprising a plurality of discrete areas; wherein
at least two of said discrete areas comprise a first discrete area and a second discrete area, said first discrete area and said second discrete area comprising a chimeric composition consisting of a nucleic acid portion covalently bound to a non-nucleic acid portion;said nucleic acid portion of the chimeric composition located at said first discrete area has a different sequence from that of said nucleic acid portion of the chimeric composition located at said second discrete area;said covalently bound non-nucleic acid portion of the chimeric composition of said first discrete area has a binding affinity for the first non-nucleic acid analyte of interest,said covalently bound non-nucleic acid portion of the chimeric composition of said second discrete area has a binding affinity for the second non-nucleic acid analyte,said first non-nucleic acid analyte is different from said second non-nucleic acid analyte, andwhen the non-nucleic acid portion is a peptide or protein, the nucleic acid portion does not comprise a sequence which is either identical or complementary to a sequence that codes for said peptide or protein; andc) signal generating means; 2) labeling said non-nucleic acid analytes of the sample with said signal generating means; 3) contacting said array with said labeled non-nucleic acid analytes under conditions permissive of binding said labeled non-nucleic acid analytes to said non-nucleic acid portion; and 4) detecting or quantifying the presence of said first and second non-nucleic acid analytes of interest by measuring said signal generating means respectively bound to said first and second discrete areas of said array.