| 摘要 |
<p>1,162,027. Antibiotic SL 2266. SANDOZ Ltd. 10 Nov., 1966 [6 Dec., 1965], No. 50518/66. Heading C2A. The invention relates to an antifungal, antitumour antibiotic SL 2266 which is an amorphous, colourless acid having the formula C 27 H 40 O 8 , a specific rotation of [α]<SP>20</SP>D = -45 degrees (C = 0.57 in ethanol), forming salts with organic or inorganic bases and showing the ultraviolet spectrum of Fig. 1, shoulder at 300 mÁ (log # = 1.84) and the infra-red spectrum of Fig. 2. The antibiotic is produced by the cultivation of stain NRRL 3196 of the fungus SORDARIA araneosa Cain or an antibiotic SL 2266 producing mutant thereof, in a nutrient medium containing an assimilable source of carbon, nitrogen and inorganic salts under surface or submerged aerobic cultivation. The antibiotic is isolated from (a) the clarified broth by extraction with a water-immiscible solvent, e.g. ethylene chloride, benzene, ethyl acetate, butyl acetate or chloroform, (b) the separated mycelium by extraction with methanol or acetone. The crude extract is extracted into water of pH 8 to 10, acidified to pH 2-5 and re-extracted with ethyl acetate. The crude antibiotic obtained after removal of the solvent is further purified by (a) chromatography, e.g. on silica gel, followed by elution with chloroform/glacial acetic acid, (b) countercurrent distribution. Examples relate to the preparation of the sodium and potassium salts. A pharmaceutical composition for internal or external use comprises the antibiotic SL 2266 or a salt thereof and a carrier.</p> |
