| 摘要 |
Elastase used in (1) food industry, for tenderising meat, (2) for tanning hides, (3) in medicine for removing necrotic skin surfaces in burns, scars or lungs, sclerotic platelets in some types of atherosclerosis, and for cleaning pus deposits, is prepared by culture of microorganism Actinomyces rimosus (A) on nutrient medium containing process for C, N and inorganic salts, sorbing the enzyme, after producer cell preparation, from soln. in presence of salicylic acid and phenol-based ion exchange resins at =10 degrees C, extracting product with a salt soln. of >=1 molar concn. and pH 9-9.4, precipitating product from eluate with an organic solvent, separating and drying. - Ion exchange resins contain salicylic acid and phenol in 1:0.2 mole ratio, and have 9-11 swelling power in Na+-form. Pref. sorption and product separation take place at 2-4 degrees C, and pref. salt extractant soln. contains 1 mole NaCl, 0.3 moles NH4Cl and NH4OH to pH 9-9.4. - (A) is producer for oxytetracycline as well, and prodn. of elastase can be combined with that of oxytetracycline.
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