| 摘要 |
<p>NEW MATERIAL:A recombinant human hepatic parenchymal cell growth factor. USE:Culture reagents for hepatic parenchymal cells, hepatic regeneration promoters, fundamental research regents for hepatic function, carcinogenic research reagents for hepatic cancer, clinical diagnostic reagents for hepatopathy and treating agent for the hepatopathy. PREPARATION:For example, rat liver RNA is extracted from a rat liver by a guanidine thiocyanate method and an mRNA is separated by an oligodT cellulose column chromatography, and used as a template to synthesize a cDNA using a reverse transcriptase, The resultant cDNA is then integrated into a plasmid, etc., to prepare a cDNA library, which is subsequently screened with a probe to select a clone containing a DNA capable of coding a human hepatic parenchymal cell growth factor. The DNA is then collected, treated with a restriction enzyme and linked to an expression vector. The obtained vector is subsequently transduced into a host cell. Transformation is carried out and the resultant transformant is cultured to afford the objective recombinant human hepatic parenchymal cell growth factor.</p> |
