Method of the detection and the quantification of Mycobacterium avium subspeceis paratuberculosis on the base of the polymer chain reaction in the real time

摘要

The present invention provides a method for molecular detection and quantification of Mycobacterium avium subspecies paratuberculosis ( MAP ) by means of real time PCR, which is based on two independent real time PCR reactions amplifying two independent loci in the MAP genome: IS 900 and F57. Both real time PCR systems are based on strategy of hybridization probes and each includes its own plasmid internal amplification control based on competitive PCR. Quantification is based on the plasmid gradient with PCR cloning products.