SYSTEM AND METHOD FOR CAPTURING AND ANALYZING CELLS

摘要

A system for isolating cells in at least one of single-cell format and single-cluster format, comprising a reservoir, including a reservoir inlet and a reservoir outlet, configured to receive a biological sample and to receive at least one fluid, a manifold configured to receive and deliver the biological sample and the at least one fluid from the reservoir into a biological sample substrate, the manifold comprising a broad surface comprising a central region configured to receive the biological sample substrate, a set of openings configured to enable fluid flow transmission across the biological sample substrate, a manifold inlet configured to transmit flow from the reservoir the first subset of openings, a manifold outlet configured at a downstream end of the broad surface and coupled to the second subset of openings, the manifold outlet configured to transmit waste fluid from the manifold.

主权项

1. A system for processing a biological sample, comprising:
a reservoir, including a reservoir inlet and a reservoir outlet, configured to receive at least one of the biological sample and a process fluid; a manifold configured to receive and deliver at least one of the biological sample and the process fluid from the reservoir to a biological sample substrate, the manifold comprising:
a broad surface comprising a central region configured to receive the biological sample substrate during operation of the system; anda set of openings defined at the central region, the set of openings comprising:
a first subset of openings configured to transmit fluid flow to the biological sample substrate; anda second subset of openings configured to receive the fluid flow from the biological sample substrate, wherein the first and the second subsets of openings are symmetrically opposed across a manifold axis; a manifold inlet configured at an upstream end of the broad surface, the manifold inlet coupled to the reservoir and to the first subset of openings, and the manifold inlet configured to transmit flow from the reservoir to the first subset of openings; and a manifold outlet configured at a downstream end of the broad surface and coupled to the second subset of openings, the manifold outlet configured to transmit waste fluid from the manifold.