METHODS AND COMPOSITIONS FOR GENERATION OF DEVELOPMENTALLY-INCOMPETENT EGGS IN RECIPIENTS OF NUCLEAR GENETIC TRANSFER

摘要

The present technology provides for a developmentally-incompetent egg cell that is produced by genetically engineering (e.g., inactivating) at least one gene in an oocyte precursor cell and culturing the oocyte precursor cell in conditions sufficient to produce an egg cell. The present technology also provides for methods of using the developmentally-incompetent egg cell.

主权项

1. A developmentally-incompetent egg cell engineered to express decreased levels, as compared to a wild-type egg cell, of one or more proteins encoded by one or more genes selected from the group consisting of: zygote arrest protein 1 (“ZAR 1”), oocyte secretory protein 1 (“OSP 1”), and maternal antigen that embryos require (“MATER”).