| 摘要 |
PURPOSE:To enable to perform a high-sensitive measurement of super oxidedismutase SOD, by using a sandwich method consisting of an antibody, enzymelabelling a SOD antibody in which copper, zinc-super cxidedismutase (SOD) or rifined homo is obtained as an antigen, and an antigen combined with an insoluble carrier. CONSTITUTION:Cu, Zn-SOD of homo is sensitized to a rabit to prepare anti- Cu, Zn-SOD antibody. The antibody is combined with an insoluble carrier, such as glass beads, to obtain an insoluble antibody. Meanwhile, a labelled antibody, in which beta-galactosidase, paraoxidase are combined with an anti-Cu, Zn-SOD antibody, is obtained. Said insoluble antibody and labelled antibody are added to a serum and urine of a patient to enable it to react to the Cu, Zn-SOD in a sample to prepare a ternary immunity complex. The measurement of fluorescence, radiated resulting from addition of the enzymer activity of the complex to a reagent, enable to perform a high-precise measurement of even a minute amount of the SOD in the sample. This enables to perform a reliable diagnosis of a kidney patient. |
