DEVICE AND METHOD FOR CELL MOTILITY ASSAY

摘要

An apparatus and method of use for assaying cellular motility in response to a concentration gradient of a chemotactic agent. Generally, the apparatus includes a chamber (6) having a region for receiving a biological sample (24) containing cells of interest and, spaced apart from such region, another region (26) for receiving a chemotactic agent. Between these regions, a concentration gradient is formed. Mapping the positions of individual cells responsive to such concentration gradient then occurs. Means for processing and analyzing the collected data are also provided. Motility determinations may be made on purified or unpurified samples. Single-site assay devices (6) and multi-site, high-throughput assay devices (36, 56, 81) are disclosed.