摘要 |
A method for measuring HCV NS3/4A activity from a HCV NS3/4A sequence, comprising obtaining and cloning the sequence into a mammalian expression vector, transiently transfecting a mammalian cell with the vector, which includes a reporter construct encoding a HCV NS3/4A cleavage site fused to a detectable label, measuring signal production from the label resulting from cleavage at the cleavage site, and measuring effects on signal production by addition of a test compound. |
申请人 |
MERCK & CO., INC.;LUDMERER, STEVEN, W.;GRAHAM, DONALD, J.;OLSEN, DAVID, B. |
发明人 |
LUDMERER, STEVEN, W.;GRAHAM, DONALD, J.;OLSEN, DAVID, B. |