发明名称 |
Method for inducing endodermal and mesodermal differentiation from human pluripotent stem cells by CXCR2 suppression |
摘要 |
The present invention relates to a method of inducing the differentiation of human pluripotent stem cells into endoderm and mesoderm by CXCR2 inhibition, and more particularly, to a method of promoting the differentiation of human pluripotent stem cells into endoderm and mesoderm by inhibiting the expression of the surface receptor CXCR2 in the stem cells by use of an shRNA technique. The method of inducing the differentiation of human pluripotent stem cells into endoderm or mesoderm by CXCR2 inhibition according to the present invention can increase the efficiency and utility of stem cells as a cell therapeutic agent, because it promotes the differentiation of stem cells into a specific germ layer serving as the origin of target cells, which is the first important step for inducing the differentiation of stem cells into specific cells. |
申请公布号 |
US9580682(B1) |
申请公布日期 |
2017.02.28 |
申请号 |
US201514936774 |
申请日期 |
2015.11.10 |
申请人 |
KOREA UNIVERSITY RESEARCH AND BUSINESS FOUNDATION |
发明人 |
Kim Byung Soo;Jung Ji-Hye;Park Yong |
分类号 |
C12N5/00;C12N5/073 |
主分类号 |
C12N5/00 |
代理机构 |
Hultquist, PLLC |
代理人 |
Hultquist, PLLC ;Hultquist Steven J. |
主权项 |
1. A method for inducing the differentiation of pluripotent stem cells into endoderm or mesoderm, the method comprising a step of either in vitro treating the pluripotent stem cells with a CXCR2 expression inhibitor or culturing the pluripotent stem cells in a medium containing a CXCR2 antagonist. |
地址 |
Seoul KR |