| 主权项 |
1. A cytometry apparatus for the analysis of stationary and flowing samples comprising:
a stage, wherein the stage is configured to receive and support a cytometry cuvette, a cytometry cuvette, wherein the cytometry cuvette is supported by and is removable from the stage, wherein the cytometry cuvette comprises a sample plane, wherein the cytometry cuvette is configured to support therein a biological sample, and wherein a biological sample supported therein is supported on the sample plane of the cytometry cuvette; an objective, wherein the objective comprises an entrance pupil and an exit pupil, and wherein the objective is optically coupled to the stage such that light from the biological sample traveling out of said sample plane may enter said entrance pupil and exit said exit pupil; a sample coplanar light source disposed in substantially the same plane as the sample plane, wherein light from said sample coplanar light source is not in optical communication with said objective; a sample coplanar optical sensor disposed substantially the same plane as the sample plane, wherein said sample coplanar optical sensor is not in optical communication with the objective; a first detection train, wherein the first detection train comprises one or more image sensors in optical communication with said objective and configured for image analysis of stationary samples; a second detection train, wherein the second detection train comprises one or more light detectors in optical communication with said objective and configured for the analysis of flowing samples; an actuatable structure for directing light, said actuatable structure having a first position and a second position, wherein the actuatable structure is coupled to an actuation mechanism and, in said first position, the actuatable structure is configured to direct light from the exit pupil of the objective to the first detection train for the analysis of stationary samples and, in said second position, the actuatable structure is configured to direct light from the exit pupil of the second detection train for the analysis of flowing samples; and an illumination train, wherein the illumination train comprises a light source in optical communication with said objective, wherein the illumination train is configured to provide at least a first illumination output and a second illumination output, and wherein for the first illumination output the illumination train is configured to focus light of a first range of wavelengths from the light source on a first location on the sample plane of the cytometry cuvette and the illumination train is configured to focus light of a second range of wavelengths from the light source on a second location on the sample plane of the cytometry cuvette. |
