发明名称 |
METHOD OF AGGLUTINATION IMMUNOASSAY |
摘要 |
The present invention provides a particle enhanced agglutination immunoassay including the steps of: mixing a sample solution containing an analyte with a solution containing insoluble carrier particles carrying a binding partner or binding partners for the analyte to prepare a mixed solution; determining a variation (i) in intensity of light scattered from the mixed solution based on a difference in intensity of scattered light between first and second time points; determining a variation (ii) in absorbance of the mixed solution based on a difference in absorbance between third and fourth time points; and correlating the determined variation (i) in intensity of scattered light and the determined variation (ii) in absorbance with an amount of the analyte present in the sample using a calibration curve plotted based on the variation in intensity of scattered light and a calibration curve plotted based on the variation in absorbance. The present invention employs measurements of the intensity of scattered light and the absorbance in combination for a single assay, and thus provides a particle enhanced agglutination immunoassay which achieves higher sensitivity and a wider dynamic range than conventional assays. |
申请公布号 |
US2016195522(A1) |
申请公布日期 |
2016.07.07 |
申请号 |
US201414894207 |
申请日期 |
2014.06.02 |
申请人 |
SEKISUI MEDICAL CO., LTD. |
发明人 |
TAKAHASHI Hiroshi;YOSHIDA Tadaaki;BANBA Yoshimasa;TAKAHASHI Yuki |
分类号 |
G01N33/543 |
主分类号 |
G01N33/543 |
代理机构 |
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代理人 |
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主权项 |
1. A particle enhanced agglutination immunoassay comprising the steps of:
mixing a sample solution containing an analyte with a solution containing insoluble carrier particles carrying a binding partner or binding partners for the analyte to prepare a mixed solution; determining a variation (i) in intensity of light scattered from the mixed solution based on a difference in intensity of scattered light between first and second time points; determining a variation (ii) in absorbance of the mixed solution based on a difference in absorbance between third and fourth time points; and correlating the determined variation (i) in intensity of scattered light and the determined variation (ii) in absorbance with an amount of the analyte present in the sample using a calibration curve plotted based on the variation in intensity of scattered light and a calibration curve plotted based on the variation in absorbance. |
地址 |
Tokyo JP |