METHODS FOR DETERMINATION OF TOTAL HOMOCYSTEINE

摘要

Disclosed herein are simple, rapid and cost effective methods for determination of endogenous sulfhydryl amino acid species from a sample, example a sample in clinical practice. The method can include mixing of a biological sample with an internal standards which does not require pretreatment or derivatization and/or isotopic dilution. The method can further include analyzing the sample using liquid chromatography tandem mass spectrometer (LC-MS/MS) to determine the concentration of sulfhydryl amino acid species.

主权项

1. A method for detection or determination of one or more different endogenous sulfhydryl amino acid species from a sample, comprising:
a) mixing the sample with an internal standard; b) reducing the sulfhydryl amino acid species in the sample by a thiol compound; c) precipitating proteins in the sample to form a mixture; d) obtaining a supernatant liquid from the mixture of step (c); and e) analyzing said supernatant using liquid chromatography tandem mass spectrometer (LC-MS-MS) to determine the sulfhydryl amino acid concentration present in the sample; wherein, the method does not include derivatization and/or isotopic dilution of the internal standard.