Method and compounds for generation of iPSCs

摘要

The present invention is based on the seminal concept of combining genomics and chemical biology to identify new agents useful for induced pluripotent stem cell (iPSC) generation. The invention provides a method of generating an iPSC utilizing agents that antagonize a cell specific gene or upregulate expression or activity of a nuclear reprogramming gene, as well as a method of screening for such agents.

主权项

1. An in vitro method of generating an induced pluripotent stem cell (iPSC) comprising:
a) contacting a human or mouse fibroblast cell with nuclear reprogramming factors, the nuclear reprogramming factors being OCT4, SOX2, KLF4 and C-MYC, wherein the nuclear reprogramming factors are encoded by one or more polynucleotides; and b) contacting the cell of (a) with an agent that inhibits COX-2 expression or activity, wherein the agent is a polynucleotide, and wherein reprogramming efficiency is increased as compared to generation of an iPSC without contacting the cell with the agent, thereby generating an iPS cell.