| 发明名称 |
METHOD FOR PRODUCING RETINAL PIGMENT EPITHELIAL CELLS |
| 摘要 |
The present invention provides
a method for producing a retinal pigment epithelial cell, including(1) a first step of subjecting pluripotent stem cells to floating culture in a serum-free medium to form an aggregate of pluripotent stem cells,(2) a second step of subjecting the aggregate formed in step (1) to floating culture in a serum-free medium or serum-containing medium each being free of a substance acting on the Sonic hedgehog signal transduction pathway and containing a substance acting on the BMP signal transduction pathway, thereby obtaining an aggregate containing retinal progenitor cells, and(3) a third step of subjecting the aggregate formed in step (2) to floating culture in a serum-free medium or serum-containing medium each being free of a substance acting on the Sonic hedgehog signal transduction pathway and a substance acting on the BMP signal transduction pathway and containing a substance acting on the Wnt signal pathway, thereby obtaining an aggregate containing retinal pigment epithelial cell. |
| 申请公布号 |
US2016264936(A1) |
申请公布日期 |
2016.09.15 |
| 申请号 |
US201415035966 |
申请日期 |
2014.10.02 |
| 申请人 |
SUMITOMO CHEMICAL COMPANY, LIMITED ;RIKEN |
发明人 |
NAKANO Tokushige;SASAI Yoshiki;OZONE Chikafumi |
| 分类号 |
C12N5/079;A61L27/38;A61K35/30 |
主分类号 |
C12N5/079 |
| 代理机构 |
|
代理人 |
|
| 主权项 |
1. A method for producing a retinal pigment epithelial cell, comprising
(1) a first step of subjecting pluripotent stem cells to floating culture in a serum-free medium to form an aggregate of pluripotent stem cells, (2) a second step of subjecting the aggregate formed in step (1) to floating culture in a serum-free medium or serum-containing medium each being free of a substance acting on the Sonic hedgehog signal transduction pathway and containing a substance acting on the BMP signal transduction pathway, thereby obtaining an aggregate containing retinal progenitor cells, and (3) a third step of subjecting the aggregate obtained in step (2) to floating culture in a serum-free medium or serum-containing medium each being free of a substance acting on the Sonic hedgehog signal transduction pathway and a substance acting on the BMP signal transduction pathway and containing a substance acting on the Wnt signal pathway, thereby obtaining an aggregate containing retinal pigment epithelial cell. |
| 地址 |
Chuo-ku, Tokyo JP |
