发明名称 |
HOMOLOGOUS RECOMBINATION IN MISMATCH REPAIR INACTIVATED EUKARYOTIC CELLS |
摘要 |
<p>A mammalian cell having a mismatch repair-deficient phenotype is provided, where one or both alleles of a gene essential for mismatch repair, such as an Msh gene, are inactivated. Using this cell in a gene knock-out methodology advantageously allows efficient homologous recombination, even when the DNA sequences of the donor and recipient sequences diverge by significantly more than 0.6%.</p> |
申请公布号 |
EP0842289(B1) |
申请公布日期 |
2004.10.20 |
申请号 |
EP19950944705 |
申请日期 |
1995.07.26 |
申请人 |
MIXIS FRANCE S.A. |
发明人 |
TE RIELE, HENRICUS, PETRUS, JOSEPH;DE WIND, NIELS;DEKKER-VLAAR, HELENA, MARIA, JOHANNA |
分类号 |
C12N15/85;C12N15/90;(IPC1-7):C12N15/90 |
主分类号 |
C12N15/85 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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