| 摘要 |
A method for preparing a protein chip is provided to reduce the preparation time of the protein chip significantly, be easy to analyze a protein at low concentration by increasing the binding affinity between proteins through modification of a medium and analyze a plurality of proteins simultaneously by maintaining the hydrophobicity of a free portion of the protein chip. A method for preparing a protein chip comprises the steps of: (a) putting a metal chip obtained by depositing the surface of a glass substrate with gold in a reaction solution including a thiol compound having a terminal acid group to fix the thiol compound on the surface of the metal chip; (b) introducing the diamine compound into the acid group terminal of the thiol compound by reacting the thiol compound-fixed metal chip with a diamine compound such as ethylenediamine, hydrazine, 1,3-diaminopropane and putrescine to prepare a raw material; (c) reacting carboxymethyl-dextran with N-hydroxysuccinimide in the presence of carbodiimide by the following reaction formula(1) to prepare N-hydroxysuccinimide-dextran as a protein reception medium; and (d) reacting the prepared raw material with the protein receptor medium to form an amide-linked N-hydroxysuccinimide-dextran(AL NHS-dextran) at an amine group terminal of the raw material. In the reaction formula(1), n is an integer of at least 1. A protein chip prepared by the method comprises: a raw material prepared by introducing a diamine compound to a terminal(-OH) of an acid group of a thiol compound having the acid group at the terminal fixed on the metal chip; and N-hydroxysuccinimide dextran which is an amide-linked protein reception medium.
|
