| 摘要 |
<p>[PROBLEMS] To provide a modified pyrroloquinoline quinone glucose dehydrogenase having a high glucose-selectivity. [MEANS FOR SOLVING PROBLEMS] Disclosed is a modified pyrroloquinoline quinone glucose dehydrogenase which has the substitution of an amino acid residue G at position-99 in a pyrroloquinoline quinone glucose dehydrogenase (PQQGDH) depicted in SEQ ID NO:1 or an amino acid residue G at position-100 in a pyrroloquinoline quinone glucose dehydrogenase (PQQGDH) depicted in SEQ ID NO:3 by an amino acid sequence TGZN [wherein Z represents SX, S or N; and X represents an arbitrary amino acid residue]. The modified PQQGDH may further have at least one mutation selected from the group consisting of Q192G, Q192A or Q192S; L193X; E277X; A318X; Y367A, Y367F or Y367W; G451C; and N452X [wherein X represents an arbitrary amino acid residue].</p> |
