摘要 |
Method and apparatus for quantitating enzyme activity in blood and other fluids, using a spot test technique. The test utilizes enzyme standards freeze-dried in absorbent discs held under a membrane filter. Other discs above the filter contain substrate, cofactors, and indicators. The spot test plates are entirely self-contained, requiring neither instrumentation nor measurement of fluid volumes, and the test can be carried out under any ambient conditions. The test is particularly applicable to emergency screening requirements, such as may occur in hospital receiving rooms, doctors' offices, ambulances, or whereever rapid diagnosis of myocardial infraction may be required.
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