发明名称 |
METHOD AND APPARATUS FOR MALDI ANALYSIS |
摘要 |
Matrix assisted laser desorption/ionization is performed in a manner to thermalize large analyte ions in a plume of desorbed material for spectroscopic analysis. The thermalized ions have a low or zero mean velocity and are presented at a well-defined instant in time, reducing artifacts and sharpening the spectral peaks. In one embodiment the light is delivered to a matrix or sample holder having a cover, baffle or compartment. The baffle or compartment impedes or contains a plume of desorbed material and the analyte undergoes collisions to lower its mean velocity and directionality. Thus "thermalized", the analyte ions are passed to a mass analysis instrument. In a preferred embodiment, an optical fiber butts up against a thin transparent plate on which the specimen resides, with the matrix side in a vacuum acceleration chamber. A mechanical stage moves the specimen in both the x- and y-directions to select a point on the specimen which is to receive the radiation. The use of a fiber optic illuminator allows the entire stage assembly to be subsumed essentially within the dimensions of a conventional stage.
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申请公布号 |
CA2237946(A1) |
申请公布日期 |
1998.03.26 |
申请号 |
CA19972237946 |
申请日期 |
1997.09.19 |
申请人 |
SEQUENOM, INC. |
发明人 |
HIGGINS, SCOTT;HILLENKAMP, FRANZ;REUTER, DIRK;LOUGH, DAVID M. |
分类号 |
G01N27/62;B01D59/44;C40B60/14;G01N27/64;H01J49/00;H01J49/04;H01J49/10;H01J49/16;(IPC1-7):H01J49/16 |
主分类号 |
G01N27/62 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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