| 摘要 |
<p>PROBLEM TO BE SOLVED: To obtain the subject optically active compound as an important synthetic intermediate of an amide type local anesthetic agent, an anticoagulant, etc., and also useful as a proline analog by asymmetrically hydrolyzing a 2- piperidine carboxylic acid amide with a specific new amidase. SOLUTION: This piperidine carboxylic acid is obtained by an asymmetric hydrolysis of an S-form 2-piperidine carboxylic acid amide of formula I (R1 , R2 are independently H or an alkyl; * represents an asymmetric carbon) as a substrate with a new amidase derived from a bacterium belonging to the genus Mycoplasma [e.g.; Mycoplasma sp. TST-027 (FERM-P-15861)]. The S-form 2-piperidine carboxylic acid of formula II is obtained by affecting the enzyme to S-form 2-piperidine carboxylic acid amide of formula I, and also an R-form 2-piperidine carboxylic acid amide of formula III can be recovered by affecting the enzyme to a mixture of the compounds of formula I and III and separating the reduced compound of the formula II.</p> |
