A Bacillus strain has a chromosome with the following modifications: a mutation of a spolllE gene which blocks transfer of the prespore chromosome; a mutation which prevents loss of SpoOJ function from blocking sporulation; a first reporter gene dependent on sigma <F> factor and placed at a location where impaired SpoOJ function leads to increased trapping in the prespore; and a second reporter gene having a promoter which is dependent on sigma <F> factor and where impaired SpoOJ function leads to reduced trapping in the prespore. The strain is useful in a method of screening for putative antibiotics.
申请公布号
WO9826087(A1)
申请公布日期
1998.06.18
申请号
WO1997GB03401
申请日期
1997.12.10
申请人
ISIS INNOVATION LIMITED;ERRINGTON, JEFFERY;WU, LING, JUAN