MONOCLONAL ANTIBODIES SELECTIVELY RECOGNIZING LISTERIA MONOCYTOGENES, HYBRIDOMA PRODUCING THE ANTIBODY, TEST KIT COMPRISING THE SAME ANTIBODY AND DETECTION METHOD USING THE SAME, THEREBY DETECTING POLLUTED FOOD BY PATHOGENIC BACTERIA

摘要

PURPOSE: Monoclonal antibodies selectively recognizing Listeria monocytogenes, hybridoma producing the antibody, a test kit comprising the same antibody and a detection method using the same antibody are provided, thereby rapidly detecting polluted food by pathogenic bacteria. CONSTITUTION: The monoclonal antibody selectively recognizing a protein p60 of Listeria monocytogenes is produced from hybridoma(KCTC 10431BP). The monoclonal antibody selectively recognizing a protein p60 of Listeria spp. is produced from hybridoma(KCTC 10432BP). The method for detecting infection of Listeria monocytogenes comprises contacting the monoclonal antibody with a sample and analyzing the presence of a complex of protein p60 of Listeria monocytogenes and monoclonal antibody by using RIA, ELISA, immune-fluorescence, pigment particle combination or chemical luminous material combination.