发明名称 |
VERFAHREN ZUR BESTIMMUNG DER NITROSYLIERUNG VON PROTEINEN |
摘要 |
Many of the effects of nitric oxide are mediated by the direct modification of cysteine residues resulting in an adduct called a nitrosothiol. A method to detect proteins which contain nitrosothiols involves several steps. Nitrosylated cysteines are converted to tagged cysteines. Tagged proteins can then be detected, for example, by immunoblotting and/or can be purified by affinity chromatography. The method is applicable to the detection of S-nitrosylated proteins in cell lysates following in vitro S-nitrosylation, as well as to the detection of endogenous S-nitrosothiols in selected protein substrates. |
申请公布号 |
DE60118621(T2) |
申请公布日期 |
2007.05.03 |
申请号 |
DE2001618621T |
申请日期 |
2001.10.29 |
申请人 |
THE JOHNS HOPKINS UNIVERSITY;MEMORIAL SLOAN-KETTERING CANCER CENTER |
发明人 |
JAFFREY, CORNELL;FERRIS, D.;SNYDER, H.;TEMPST, PAUL |
分类号 |
G01N33/68 |
主分类号 |
G01N33/68 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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