Novel Skin Remodeling Strategy

摘要

The present invention relates to a method for identifying materials having an efficacy for improving collagen turnover in the skin. The invention also concerns a composition containing a combination of a collagen-degrading active and a collagen synthesis-stimulating active for enhancing skin remodeling.

主权项

1. A method for identifying a material having an efficacy for enhancing the activity of a collagen promoting active, comprising the steps of:
(a) incubating human dermal fibroblasts (HDFs) in the presence or absence of a test material; (b) thereafter, removing the test material and incubating the HDFs in the presence of a collagen-promoting active for a time sufficient to stimulate collagen release by HDFs; and (c) measuring an amount of collagen released by HDFs treated with the test material and with the collagen promoting active and comparing that amount with an amount of collagen released by HDFs treated with the collagen-promoting active in the absence of the test material.