| 摘要 |
A plurality of liquid mobile phases stored in a plurality of reservoirs respectively are filled and pumped by a dual pump driven by a stepping motor, through valves complementarily subjected to on-off control by a gradient programmer. The dual pump has two pistons in contact with the periphery of a cam in opposition to each other at 180 degrees, which pistons fill and pump the liquid mobile phases by linear reciprocation along the contour of the cam with the rotation of the cam. The radius of the cam linearly increases in the range from 0 to 180 degrees and linearly decreases in the range from 180 to 360 degrees at the same rate as when it increases. An electric signal is produced from a timing device during a period from the rotational positions of the cam reaches 0 and 180 degrees to a predetermined rotational positions. The liquid mobile phase delivered from the dual pump is supplied to a pressure transducer, and after passing through it, supplied to a column through an injector for injecting a specimen. The components into which the specimen is separated are detected by a detector. When the timing device detects that the cam is positioned in intervals from 0 and 180 degree to a predetermined angle therefrom, the motor control circuit drives the stepping motor at high speed during period from a time which the time derivative of the back pressure signal from the pressure transducer reaches value smaller than a predetermined value to a time it reaches value larger than the predetermined value.
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