发明名称 |
METHOD FOR DETECTING A TARGET NUCLEIC ACID |
摘要 |
A method for detecting a target nucleic acid, which method includes the steps (i) amplifying the target nucleic acid to obtain an amplification product using a polymerase, a first primer with or without a segment noncontiguous to a first priming sequence, and a second primer with or without a segment noncontiguous to a second priming sequence in the presence of an oligonucleotide which is incapable of acting as a primer for the polymerase, wherein the oligonucleotide has at least 5 consecutive nucleotides fully complementary to at least 5 consecutive nucleotides of the first primer; and (ii) detecting the presence of the target nucleic acid by monitoring amplification thereof. In particular, the figure illustrates the method wherein the primer contains a segment noncontiguous to its priming sequence. |
申请公布号 |
WO9532306(A1) |
申请公布日期 |
1995.11.30 |
申请号 |
WO1994US05767 |
申请日期 |
1994.05.23 |
申请人 |
BIOTRONICS CORPORATION |
发明人 |
WANG, CHANG-NING, J.;WU, KAI-YUAN |
分类号 |
C12N15/09;C07H;C07H21/04;C12P;C12P19/34;C12Q;C12Q1/68;G01N33/533;G01N33/68 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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