A PROCESS FOR PREPARING ALPHA-GLYCOSIDASE INHIBITORS FROM SILKWORM URINE

摘要

PURPOSE: An α-glycosidase inhibition material from silkworm urine is provided, which shows nearly same α-glycosidase inhibition effect as the existing diabetes medicine, the Acarbose, thereby contributing to increase of farm income and enhancement of national health. CONSTITUTION: A process for the preparation of α-glycosidase inhibition material from silkworm urine comprises the steps of: filtering the silkworm urine, freeze-drying to get powder, and adding methanol(alcohol-precipitation); processing anion-exchange column chromatography with the methanol-soluble part to get active part; separating into methanol-soluble part and insoluble part to remove inorganic material in silkworm urine; adding water and acetonitrile to methanol-soluble part to separate into acetonitrile layer and water layer, and concentrating the water layer and separating into A, B and C fractions(1-deoxynojirimycin, fagomine, 1,4-dideoxy-1,4-imino-D-araviniton showing excellent α-glycosidase inhibition effect).