斑节对虾延伸因子基因序列

摘要

本发明公开了一种斑节对虾延伸因子基因，以近源物种延伸因子的基因的CDS序列的保守区设计的兼并引物，并用PCR法扩增，结合RACE技术克隆到斑节对虾延伸因子基因的全表达序列。对于研究对虾体内的延伸因子的基因结构组成，探讨其行使调控能力的功能区域位置及与其他动物的区别，从而对于研究其重组蛋白在生产中的应用提供重要的理论依据。

主权项

1、一种斑节对虾延伸因子基因，其特征在于具有以下RNA核苷酸及相应的氨基酸序列：ccttttcggcgtttccagcacacgcctccactgtgtcgtttactgagtggacttttacca60acaaacatccaagatg gtg aac ttc aca gtg gaa gaa atc cgt gag ttg 109 Met Val Asn Phe Thr Val Glu Glu Ile Arg Glu Leu 12 1 7 12atg gac aag cgg agg aac atc cgt aac atg tcc gtc att gct cac gtt 157Met Asp lys Arg Arg Asn Ile Arg Asn Met Ser Val Ile Ala His Val 2813 19 25gac cac gga aaa tct acc ctg acc gac tcc ctt gta tcg aag gcg ggt 205Asp His Gly Lys Ser Thr Leu Thr Asp Ser Leu Val Ser Lys Ala Gly 4429 35 41att att gct tcg tct cgg gca gga gaa acc cgc ttt act gat acc aga 253Ile Ile Ala Ser Ser Arg Ala Gly Glu Thr Arg Phe Thr Asp Thr Arg 6045 51 57aaa gat gaa cag gag cga tgc att acc atc aag tct act gcc atc tct 301Lys Asp Glu Gln Glu Arg Cys Ile Thr Ile Lys Ser Thr Ala Ile Ser 7661 67 73atg tac ttc aag ctg agt gac gag aac ctc gcc ctc att aac agc cct 349Met Tyr Phe Lys Leu Ser Asp Glu Asn Leu Ala Leu Ile Asn Ser Pro 9277 83 89gac caa aag gag gct ggt gaa tct ggt ttc ctt gtc aac ctt att gat 397Asp Gln Lys Glu Ala Gly Glu Ser Gly Phe Leu Val Asn Leu Ile Asp 10893 99 105tcc cct ggt cac gtt gat ttc tcc tct gag gta aca gct gcc ctc cgt 445Ser Pro Gly His Val Asp Phe Ser Ser Glu Val Thr Ala Ala Leu Arg 124109 115 121gta act gat ggt gcc ctc gtc gtg gtg gat tgc gtc tct ggt gtg tgt 493Val Thr Asp Gly Ala Leu Val Val Val Asp Cys Val Ser Gly Val Cys 140125 131 137gtg cag acc gag act gta ctg cgt cag gct att gct gag cgt atc aag 541Val Gln Thr Glu Thr Val Leu Arg Glu Ala Ile Ala Glu Arg Ile Lys 156141 147 153cca gtt ctg ttc atg aac aag atg gac cgt gct ctc ctt gaa ttg cag 589Pro Val Leu Phe Met Asn Lys Met Asp Arg Ala Leu Leu Glu Leu Gln 172157 163 169ctt gag cag gag gaa ttg tac cag aca ttc cag cgt att gtt gag aac 637Leu Glu Gln Glu Glu Leu Tyr Gln Thr Phe Gln Arg Ile Val Glu Asn 188173 179 185gtg aac gtc atc att gcc act tac aac gac gac aca ggc ccc atg ggt 685Val Asn Val Ile Ile Ala Thr Tyr Asn Asp Asp Thr Gly Pro Met Gly 204189 195 201gag atg cgt gtt gac ccc agc aag gga tct gtt gga ttt ggt tca gga 733Glu Met Arg Val Asp Pro Ser Lys Gly Ser Val Gly Phe Gly Ser Gly 220205 211 217ctc cac gga tgg gcc ttc tcc gtc aag gaa ttt gcc gac atc tac tca 781Leu His Gly Trp Ala Phe Ser Val Lys Glu Phe Ala Asp Ile Tyr Ser 236221 227 233aac atg ttc aag gtt cct gct gcc aag ctc atg aac aag ctc tgg ggc 829Asn Met Phe Lys Val Pro Ala Ala Lys Leu Met Asn Lys Leu Trp Gly 252237 243 249gag aac ttc ttc aac aag aag aca aag aag tgg tcc acg aac aag agc 877Glu Asn Phe Phe Asn Lys Lys Thr Lys Lys Trp Ser Thr Asn Lys Ser 268253 259 265act gat aat gag cgt gcc ttc aat aca tac atc ttg gac ccc att ttc 925Thr Asp Asn Glu Arg Ala Phe Asn Thr Tyr Ile Leu Asp Pro Ile Phe 284269 275 281aag ctc ttt gat gct atc atg aac ttc aag aaa gag gaa act gcc aaa 973Lys Leu Phe Asp Ala Ile Met Asn Phe Lys Lys Glu Glu Thr Ala Lys 300285 291 297ctg ttg gag acc ctt cag atc aag ctt caa gta gat gac agg gag aaa 1021Leu Leu Glu Thr Leu Gln Ile Lys Leu Gln Val Asp Asp Arg Glu Lys 316301 307 313gaa gga aag gcc ctc ctc aag gtt gtt atg cga act tgg cta cct gct 1069Glu Gly Lys Ala Leu Leu Lys Val Val Met Arg Thr Trp Leu Pro Ala 332317 323 329gga gac acg ctc ttc cac atg atc acc att cac ctt cca tcc cct gtg 1117Gly Asp Thr Leu Phe His Met Ile Thr Ile His Leu Pro Ser Pro Val 348333 339 345act gcc cag aaa tac cgt gct gag atg ctg tac gaa ggt cca tct gat 1165Thr Ala Gln Lys Tyr Arg Ala Glu Met Leu Tyr Glu Gly Pro Ser Asp 364349 355 361gac ctt gct tgc act ggc atc aag aac tgc gat tct gat gct cct ctt 1213Asp Leu Ala Cys Thr Gly Ile Lys Asn Cys Asp Ser Asp Ala Pro Leu 380365 371 377atg atg tac gtg tca aag atg gta cca aca tct gac aag ggt cgc ttc 1261Met Met Tyr Val Ser Lys Met Val Pro Thr Ser Asp Lys Gly Arg Phe 396381 387 393tat gcc ttc ggt cgt gtc ttt gcc ggc aag gtt ggc agt ggt cag aag 1309Tyr Ala Phe Gly Arg Val Phe Ala Gly Lys Val Gly Ser Gly Gln Lys 412397 403 409gtc cgc atc atg ggt ccc aac tac gtc cca gga aag aag gaa gat ctc 1357Val Arg Ile Met Gly Pro Asn Tyr Val Pro Gly Lys Lys Glu Asp Leu 428413 419 425ttc gag aag gcc atc cag aga acc att ctt atg atg ggt cgc ttt gtt 1405Phe Glu Lys Ala Ile Gln Arg Thr Ile Leu Met Met Gly Arg Phe Val 444429 435 441gaa gct att gag gat gtc cct gct ggt aac att tgt ggt ctt gtt ggt 1453Glu Aal Ile Glu Asp Val Pro Ala Gly Asn Ile Cys Gly Leu Val Gly 460445 451 457gtt gac cag tac ctt gtt aag act ggt acc atc act acc agc aag gac 1501Val Asp Gln Tyr Leu Val Lys Thr Gly Thr Ile Thr Thr Ser Lys Asp 476461 467 473tcc cac aac atg aag gtg atg aag ttc agt gtg tcg cct gtc gtg cgt 1549Ser His Asn Met Lys Val Met Lys Phe Ser Val Ser Pro Val Val Arg 492477 483 489gtg gct gtt gaa ccc aag aac cca tct gac ttg ccc aag ctt gtg gaa 1597Val Ala Val Glu Pro Lys Asn Pro Ser Asp Leu Pro Lys Leu Val Glu 508493 499 505ggt ctc aaa cgt ctt tcc aag tct gac cct atg gtg caa tgt atc att 1645Gly Leu Lys Arg Leu Ser Lys Ser Asp Pro Met Val Gln Cys Ile Ile 524509 515 521gag gaa tct ggt gag cac atc att gct gga gct ggt gaa ctt cat ctt 1693Glu Glu Ser Gly Glu His Ile Ile Ala Gly Ala Gly Glu Leu His Leu 540525 531 537gag att tgt ctg aag gat ctt gaa gaa gac cat gct tgc att cct ctc 1741Glu Ile Cys Leu Lys Asp Leu Glu Glu Asp His Ala Cys Ile Pro Leu 556541 547 553aag aag acc gac cca gtt gta tcc tac agg gag acg gtt agt gct cct 1789Lys Lys Thr Asp Pro Val Val Ser Tyr Arg Glu Thr Val Ser Ala Pro 572557 563 569tca act gag ctt tgt cta tcc aag tcc ccc aac aag cac aac cgt ctg 1837Ser Thr Glu Leu Cys Leu Ser Lys Ser Pro Asn Lys His Asn Arg Leu 580573 579 585tac atg agg gcc gtg cct atg cct gat gga ctt gct gat gac att gag 1885Tyr Met Arg Ala Val Pro Met Pro Asp Gly Leu Ala Asp Asp Ile Glu 604589 595 601gct ggt aaa gta act ccc cgt gat gac ccc aag acc agg aag tca tac 1933Ala Gly Lys Val Thr Pro Arg Asp Asp Pro Lys Thr Arg Lys Ser Tyr 620605 611 617ctg tgt gag cac tac gag ttc gat gcc act gat gcc atg aaa atc tgg 1981Leu Cys Glu His Tyr Glu Phe Asp Ala Thr Asp Ala Met Lys Ile Trp 636621 627 633act ttc ggc cct gaa tct act gga ggc aac atc ttg gtt gat gta aca 2029Thr Phe Gly Pro Glu Ser Thr Gly Gly Asn Ile Leu Val Asp Val Thr 652637 643 649aag gga gtg cag tac ctc aat gaa atc aag gat tct tgc gta gct ggc 2077Lys Gly Val Gln Tyr Leu Asn Glu Ile Lys Asp Ser Cys Val Ala Gly 668653 659 665ttc cag tgg gca acc aag gaa ggt gtc ctt tgc gac gag aac atg cgt 2125Phe Gln Trp Ala Thr Lys Glu Gly Val Leu Cys Asp Glu Asn Met Arg 684669 675 681agc gtc cgt ttc aac ctc cat gat gta acc ctc cat gct gat gct atc 2173Ser Val Arg Phe Asn Leu His Asp Val Thr Leu His Ala Asp Ala Ile 700685 691 697cat cgt ggt ggc ggc cag atc att ccc acc act cga cgt gtg ctg tac 2221His Arg Gly Gly Gly Gln Ile Ile Pro Thr Thr Arg Arg Val Leu Tyr 716701 707 713gct agt gtc ctc aca gct gag ccc cgt ctt cag gag cca gta tac ctc 2269Ala Ser Val Leu Thr Ala Glu Pro Arg Leu Gln Glu Pro Val Tyr Leu 732717 723 729tgt gag atc cag tgt cct gag gct gcc gtc ggc ggt atc tat ggt gta 2317Cys Glu Ile Gln Cys Pro Glu Ala Ala Val Gly Gly Ile Tyr Gly Val 748733 739 745ctc aac agg cgt cga ggt gtt gtg ttc gag gag aac cag gtt gct ggt 2365Leu Asn Arg Arg Arg Gly Val Val Phe Glu Glu Ash Gln Val Ala Gly 764749 755 761acg cct atg ttc gtt gtc aaa gct cat ctt cct gtc aac gag tcc ttc 2413Thr Pro Met Phe Val Val Lys Ala His Leu Pro Val Asn Glu Ser Phe 780765 771 777ggc ttc act gca gat crt cgc tcc aac aca ggt gga cag gcc ttc cca 2461Gly Phe Thr Ala Asp Leu Arg Ser Asn Thr Gly Gly Gln Ala Phe Pro 796781 787 793cag tgc gtg ttc gat cac tgg caa ggg atg ccc ggc aat ccc atg gat 2509Gln Cys Val Phe Asp His Trp Gln Gly Met Pro Gly Asn Pro Met Asp 812797 803 809acc act ggc agc agc aaa ccc tac acc atc gtt tgc gat acg agg aag 2557Thr Thr Gly Ser Ser Lys Pro Tyr Thr Ile Val Cys Asp Thr Arg Lys 828813 819 825agg aag ggc ctg aaa gaa ggc ctt ccc gat tta gcc aac tac ctg gac 2605Arg Lys Gly Leu Lys Glu Gly Leu Pro Asp Leu Ala Asn Tyr Leu Asp 844829 835 841aag ctg taa 2614Lys Leu 846845tcacaatattcacataccatagatatataatcctaaggttttatttactatatatgttac 2674ggtgattacaataaagtggaagggagtcgcaaaaaaaaaaaaaaaaaaaaaa 2726