| 主权项 |
1. A method of detecting the presence of a coronavirus in a sample and identifying the subgroup of the coronavirus in the sample, comprising:
a) contacting a sample with a panel of proteins comprising: 1) one or more nucleocapsid proteins from a subgroup 2c coronavirus, 2) one or more nucleocapsid proteins from a subgroup 2b coronavirus, 3) one or more nucleocapsid proteins from a subgroup 2a coronavirus, 4) one or more nucleocapsid proteins from a subgroup 2d coronavirus, 5) one or more nucleocapsid proteins from a subgroup 1a coronavirus, 6) one or more nucleocapsid proteins from a subgroup 1b coronavirus, and 7) any combination of (1) through (6) above, under conditions whereby an antigen/antibody complex can form; and b) detecting formation of an antigen/antibody complex, whereby detection of formation of the antigen/antibody complex detects a coronavirus in the sample and whereby detection of formation of an antigen/antibody complex comprising the nucleocapsid protein(s) of (1) identifies the subgroup of the coronavirus in the sample as subgroup 2c; detection of an antigen/antibody complex comprising the nucleocapsid protein(s) of (2) identifies the subgroup of the coronavirus in the sample as subgroup 2b; detection of an antigen/antibody complex comprising the nucleocapsid protein(s) of (3) identifies the subgroup of the coronavirus in the sample as subgroup 2a; detection of an antigen/antibody complex comprising the nucleocapsid protein(s) of (4) identifies the subgroup of the coronavirus in the sample as subgroup 2d; detection of an antigen/antibody complex comprising the nucleocapsid protein(s) of (5) identifies the subgroup of the coronavirus in the sample as subgroup 1a; and detection of an antigen/antibody complex comprising the nucleocapsid protein(s) of (6) identifies the subgroup of the coronavirus in the sample as subgroup 1b. |
