| 摘要 |
PURPOSE:To obtain the subject compound having low viscosity and high anticariogenicity and useful for sweetener, etc., by reacting a high-concentration sucrose solution with dextran synthase under specific condition and reacting the product with a high-concentration sucrose solution and a solution containing dextran synthase. CONSTITUTION:The objective low-molecular dextran having an average molecular weight of about 2,000-100,000 and useful as a sweetener having high anticariogenicity, a raw material for a plasma increasing agent, a raw material for various foods, etc., is produced by culturing Leuconostoc mesenteroides strain NRRL B-512 in a medium, removing the bacterial cells by the centrifugal separation of the culture liquid, adding ethyl alcohol to the culture liquid at a low temperature, separating the precipitate to obtain a dextran synthase, adding this enzyme to a high-concentration sucrose solution and reacting at pH4-8 and 10-40 deg.C to perform the 1st stage synthesis reaction, adding an additional high-concentration sucrose solution and a solution containing dextran synthase to the reaction liquid of the 1st stage reaction and carrying out the 2nd stage reaction of the solutions one or more times. |
