| 摘要 |
<p>PURPOSE:To provide the subject system and method capable of continuously performing suspension perfusion culture in a favorable state even for adherable or spendable cells with nature liable to form cell aggregation while selectively eliminating such cell aggregates formed. CONSTITUTION:A culture tank body 1 equipped with an agitating means 37 is sterilely connected, via respective pumps 11,11, to a temporary cell reservoir 9 and a waste liquor tank 10. During culture, agitation is temporarily stopped to sediment cell aggregates developed, and a supernatant culture fluid containing proper cells to be recovered is put to suction into the reservoir 9 where the culture fluid is temporarily reserved and cell aggregates stuck to the tank wall are debonded by such means as vigorous agitation or application of vibratory ultrasonic waves and discharged, together with cell aggregates left in the culture tank, into the waste liquor tank 10. Then, the proper cells are returned to the culture tank and culture operation is continued while eliminating cell aggregates, resulting in selection of necessary amount of such fine proper cells which are left in the culture tank, thus enabling material production by continuous favorable perfusion culture of cells with nature liable to form cell aggregates.</p> |
