| 摘要 |
1295219 Photo-electric analysis of milk samples TECHNICON INSTRUMENTS CORP 19 April 1971 [29 Jan 1970] 20686/71 Heading G1A [Also in Division B1] A method of determining the number of somatic cells in a milk sample comprises mixing with the milk sample a fixative capable of inhibiting lysis and solubilization of the somatic cells, clarifying the milk and solubilizing the fatty and other non- cellular constituents to leave the somatic cells as sole insolubilized constituent and counting the number of somatic cells in the clarified milk sample. In the apparatus shown, milk samples from containers 24 interspersed with wash liquid samples from 26 are mixed at the junction of lines 43, 39, 50 with fixative reagent from 114 and at the junction of lines 52, 53 with a reagent which is a mixture of a saponifving reagent, a chelating agent and a dispersant and is fed as a mixture or as its separate components from 116, the treated milk sample passing through mixing coils 47, 48, heating bath 54 and debubbler junction 58 to the sample particle flow cell 84 which has precisely dimensioned inspection volume 84. The counting apparatus comprises light source 70, focusing means 72, collecting lenses 88, 90, dark stop 92 and photo-multiplier tube 94. The pulse output from photo-multiplier tube 94 is applied to pulse shaping circuit 98 and a rate circuit 100 and the rate output of circuit 100 is applied to recorder means 20. |
