Immunostimulant, carcinostatics TF-2 by cultivation of fusobacterium - esp. F. nucleatum, under anaerobic conditions, pptn. of supernatant with hydrophilic solvent and fractionation by Ph and protein removal

摘要

Prodn. of carcinostatic substances, TF-2, comprises cultivation of TF-2 producing bacterial strains of the Fusobacterium family and recovery of TF-2 from the culture or from the supernatant liq. New substances described are designated TF-210, 220, 230, 240, 300 (310,320,330) 340 and 350. Used as carcinostatic cpds. and immuno stimulants which may be administered orally, rectally or parenterally, pref. by injection in daily of 0.005-50 mg/kg for adults. Activity tests against Ehrlich ascitic tumours, Sarcoma 180, Ehrlich solid tumour and melanoma B-16 are described. The cpds. are particularly effective against solid tumours. Toxicity tests in mice gave intravenous LD50 values of above 200 mg/kg for TF-220, 240, 250, 340 and 350, above 100 mg/kg for TF-210 and 230 and above 10 mg/kg for TF-300.