| 摘要 |
A process for preparing pliable soft tissue specimen which are resistant to cracking and devoid of viable cells includes the steps of treating native so ft tissue obtained from a donor by a gradually increasing gradient of aliphatic alcohol or other suitable water miscible polar organic solvent until the las t alcohol (or other solvent) solution has at least 25 % by volume of the organ ic liquid. Thereafter, the tissue specimen is treated with a solution containin g glycerol or low molecular weight ( < 1000D) polyethylene glycol, and polyethylene glycol of a molecular weight between approximately 6,000 to 15,000 D and heparin. Thereafter, the tissue specimen is briefly immersed in aqueous heparin solution, frozen and lyophilized. The tissue specimen is suitable for implantation as a homograft or xenograft, with or without rehydration. |
