| 摘要 |
PROBLEM TO BE SOLVED: To provide a novel antiinflammatory agent. SOLUTION: The HAEC is separated from the placenta at the Cesarean section on the basis of the informed consent through the known procudures and cultured in the RPMI medium including 10% FCS until the HAEC is sufficiently proliferated. Subsequently, the proliferated HAEC is washed with PBS, and cultured in DMEM including 0.5% FCS for 48 hours and the supernatant is collected therefrom. When this supernatant is dropped to an eye of a mouse having the heat-coagulated central surface layer of the corneal membrane, both of the migration of Langerhans' cells in an arborous shape and the inflammatory cytokine have been found to be inhibited. |
