METHOD FOR MASS PROPAGATION OF CALLUS AND METHOD FOR MASS DIVISION OF IRIS ODAESANENSIS FLOWER UTILIZING LEAF SEGMENTS OF IRIS ODAESANENSIS

摘要

PURPOSE: A method for massively proliferating Iris odaesanensis callus and a method for massively differentiating Iris odaesanensis are provided to contribute resource conservation. CONSTITUTION: A method for massively proliferating Iris odaesanensis callus comprises: a step of extracting leaf fragment of the Iris odaesanensis callus; a step of culturing in MS(Murashige & Skoog) culture medium to induce callus; and a step of inoculating the induced callus in MS liquid medium containing sucrose and growth hormone containing 3mg/L of NAA(1-Naphthaleneacetic acid]) and 5mg/L of Ba(6-benzyladenine) then culturing in shaker to proliferate callus.